Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-12-29
1998-11-03
Railey, II, Johnny F.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 911, 4351723, 4352523, 43525233, 4353201, 536 241, C12N 121, C12N 1564, C12P 1934, C12P 2100
Patent
active
058306900
ABSTRACT:
The invention relates to the construction and use of novel expression systems that make use of DNA sequences encoding a promoter or promoters along with cis-regulatory elements, such as those for proU, that permit osmotically inducible initiation of transcription in bacteria; the expression systems of the present invention can be used to efficiently hyperexpress heterologous gene products including proteins or polypeptides; methods for construction of vectors and strains enabling NaCl-induced hyperexpression of heterologous gene products in several organisms such as Escherichia coli, and methods for NaCl-induced hyperexpression of heterologous gene products, including proteins or polypeptides, using the said novel combinations in a variety of organisms such as Escherichia coli, and further purification of the gene product of interest in either laboratory or industrial scale production systems.
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Bhandari Poonam
Gowrishankar Jayaraman
Rajkumari Kaveti
Council of Scientific & Indus. Res. & Dept. of Biotech.
Railey II Johnny F.
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