Process for producing physiologically active substance

Details Process for producing physiologically active substance Process for producing physiologically active substance

1986-05-13

1991-03-19

Warren, Charles F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Process involving micro-organisms of different genera in the...

435 41, 435 89, 435 92, 435 3, 435813, C12P 3900, C12P 100, C12P 1930, C12Q 300

Patent

active

050010557

ABSTRACT:
A process for producing a physiologically active substance by a combined enzymatic method is disclosed. In the combined enzymatic method, a reactant solution containing a precursor or precursors for the physiologically active substance, AXP, and a divalent metal ion is supplied at one end of a reactor incorporating either the combined enzymatic reaction system (a) or (b), wherein (a) is a reaction system including an enzyme for converting AMP to ADP, an enzyme for converting ADP to ATP, and an enzyme which catalyzes the synthesis of the physiologically active substance as it converts ATP to AMP; and (b) is a reaction system including an enzyme for converting ADP to ATP and an enzyme which catalyzes the synthesis of the physiologically active substance as it converts ATP to ADP, wherein the concentration of the divalent metal ion supplied into the reactor is held at a level no higher than 30 mM while the concentration of the AXP is held below that of that of the precursor or precursors for the physilogically active substance, and the physiologically active substance produced is withdrawn from the other end of the reactor.

REFERENCES:
Pollock et al, "Large-Scale Enzymatic Synthesis with Cofactor Regeneration: Glucose-6-Phosphate", J. Am. Chem. Soc. 99, 2366-2367, 1977.
Kondo et al, "Construction of a System for the Regeneration of Adenosine-5'-Triphosphate, Which Supplies Energy to Bioreactor", J. Applied Biochemistry, 1984, vol. 6, 29-38.

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