Process for producing physiologically active protein using...

Details Process for producing physiologically active protein using... Process for producing physiologically active protein using...

2003-03-06

2010-02-09

Sajjadi, Fereydoun G (Department: 1633)

Chemistry: molecular biology and microbiology

Vector, per se

C800S021000, C800S004000

Reexamination Certificate

active

07659112

ABSTRACT:
The present invention provides a genetic engineering material for insects that enables a target protein to be purified easily, without requiring the use of recombinant baculovirus, while simultaneously providing a process for producing exogenous protein using that genetic engineering material. A gene recombinant silkworm is obtained by inserting an exogenous protein gene such as a cytokine gene coupled to a promoter that functions in silk glands into a silkworm chromosome. An exogenous protein such as a cytokine is then extracted and purified from the silk glands or cocoon of that silkworm or its offspring. A large amount of exogenous protein can be produced within silk gland cells, outside silk gland cells or in silk thread or a cocoon by inserting an expression gene cassette, in which the DNA sequence of the 3′ terminal portion and the DNA sequence of the 5′ terminal portion of fibroin H chain gene are fused to the exogenous protein gene, into silk gland cells and so forth.

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