Process for producing nerve stem cells, motor neurons, and...

Details Process for producing nerve stem cells, motor neurons, and... Process for producing nerve stem cells, motor neurons, and...

2007-11-13

2007-11-13

Romeo, David S (Department: 1647)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S325000, C435S354000, C435S365000, C435S368000, C530S399000

Reexamination Certificate

active

10472490

ABSTRACT:
The present invention provides a method for producing motor neurons and GABAergic neurons characterized by including suspension-culturing embryonic stem cells in the presence or absence of a protein noggin to form embryoid bodies, selectively amplifying into neural stem cells from them by suspension culture in the presence of a fibroblast growth factor and a sonic hedgehog protein, and then differentiating the same. According to this method, at least motor neurons and GABAergic neurons can be systemically and efficiently produced from ES cells. Selective acquisition of neurons would be applicable to transplant therapy for amyotrophic lateral sclerosis, Huntington's chorea, Alzheimer's disease, etc.

REFERENCES:
patent: 2003/0211605 (2003-11-01), Lee et al.
Finley et al. Journal of Neurobiol., Sep. 5, 1999, 40(3):271-87.
K.S. O'Shea et al.: “Noggin induces a neural phenotype in ES cells, which is antagonized by EMP-4” Society for Neuroscience, vol. 24, Nos. 1-2, p. 1526 1998.
Sang-Hun Lee et al.: “Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells” Nature Biotechnology, vol. 18, No. 6, pp. 675-677 Jun. 2000.
Tsutomu Nohno et al.: “Involvement of the sonic hedgehog genein chick feather formulation” Biotechnical and Biophysical Research Communications, vol. 206, No. 1, pp. 33-39 Jan. 5, 1995.
Streit, A., et al., “Chordin regulates primitive streak development and the stability of induced neural cells, but is not sufficient for neural induction in the chick embryo,” Development 125, 507-519, 1998.

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