Process for producing natural cis-3-hexenol from unsaturated fat

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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435147, 435192, C12P 702, C12P 724

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056208790

ABSTRACT:
The present invention relates to a process for producing cis-3-hexen-1-ol from an unsaturated fatty acid, in which said synthesis is carried out from the latter by the combined action of a natural system of enzyme(s) allowing the oxidation of said fatty acid to cis-3-hexenal and of a yeast allowing the reduction of cis-3-hexenal to cis-3-hexenol in a culture medium. According to a first characteristic of the present invention, the enzymatic system consists of a plant mass obtained by grinding leaves harvested whole without predilacerating them. According to a second characteristic of the present invention, the enzymatic system is introduced in the form of a ground cellular product obtained by grinding followed by cell disintegration. Finally, advantageously, a reagent chosen from a ferrous cation, acetylsalicylic acid, chlorophyll B and the enzyme catalase, makes it possible to increase the yields of cis-3-hexenol.

REFERENCES:
patent: 4769243 (1988-09-01), Kanisawa et al.
patent: 4806379 (1989-02-01), Goers et al.
patent: 5464761 (1995-11-01), Muller et al.
Biotech Abs. 95-15887 Holtz et al WO9526413 Oct. 5, 1995.

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