Process for producing monoclonal antibodies reactive with human

Details Process for producing monoclonal antibodies reactive with human Process for producing monoclonal antibodies reactive with human

1981-12-15

1985-06-11

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351722, 435240, 435 7, 435948, 436548, 436813, 424 85, 424 11, 935 93, 935104, 935107, 935110, C12P 2100, C12N 500, C12N 1500, G01N 3354, A61K 4900, A61K 3900, C12R 191

Patent

active

045229180

ABSTRACT:
Monoclonal antibodies demonstrating a reactivity with human breast cancer are produced. The hybridoma cultures secreting immunoglobins are produced by hydridoma technology. Splenic lymphocytes of mice, immunized with membrane-enriched fractions of metastatic human mammary carcinoma tissue are fused with the NS-1 non-immunoglobulin-secreting murine myeloma cell line. Screening of immunoglobulin reactivities and double cloning of cultures yielded 11 monoclonal antibodies that demonstrated activities with the surface of human mammary tumor cells and not with the surface of apparently normal human tissues. These monoclonal antibodies aid in the diagnosis, prognosis and treatment of human breast cancer.

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Sloane et al, "Distribution of Epithelial Membrane Antigen in Normal and Neoplastic Tissues and its Value in Diagnostic Tumor . . . ", Cancer, 47(4), (1981), pp. 1786-1795.
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