Process for producing microbial transglutaminase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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4353201, 4352523, 43525233, 536 232, C12N 121, C12N 910, C12N 1554, C12N 1563

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active

060134984

ABSTRACT:
Disclosed are a protein having a transglutaminase activity, which comprises a sequence ranging from serine residue at the second position to proline residue at the 331st position in an amino acid sequence represented by SEQ ID No. 1 wherein the N-terminal amino acid of the protein corresponds to serine residue at the second position of SEQ ID No. 1, a DNA encoding the protein, a transformant having the DNA, and a process for producing a protein having a transglutaminase activity, which comprises the steps of culturing the transformant in a medium. The protein can be produced in a large amount with the transformant using a host such as E. coli.

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Koji Ikura, et al., Biochemistry, pp. 2898-2905, "Amino Acid Sequence of Guinea Pig Liver Transglutaminase from its CDNA Sequence", 1998.
M.A. Phillips, et al., Proc. National Academy of Science USA, vol. 87, pp. 9333-9337, "Primary Structure of Keratinocyte Transglutaminase", Dec. 1990.
Akitada Ichinose, et al., Biochemistry, pp. 6900-6906, "Amino Acid Sequence of the A Subunit of Human Factor XIII", 1986.
A. Ben-Bassat, et al., Nature, vol. 326, p. 315, "Amino-Terminal Processing of Proteins", Mar. 19, 1987.

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