Process for producing L(-)-tetrahydrofolic acid

Details Process for producing L(-)-tetrahydrofolic acid Process for producing L(-)-tetrahydrofolic acid

1989-08-15

1990-05-29

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435119, 435177, 435191, 43525233, 435280, 435320, 435814, 435849, C12P 1304, C12N 906

Patent

active

049295519

ABSTRACT:
The invention relates to a process for producing L(-)-tetrahydrofolic acid which comprises allowing dihydrofolate reductase to act upon dihydrofolic acid in the presence of (1) NADP or NADPH, and (2) glucose and glucose dehydrogenase. L(-)-tetrahydrofolic acid is useful as the intermediate for L(-)-leucovarin.

REFERENCES:
Biotech Abs 89-15096 Ottolina et al "Biochim Biophys Acta" (1989) 998, 2, 173-178.
Chem Abs 103, 174553(21) Charlton et al "J. Chem Soc" (7) pp. 1349-1353 (1985).
J. Medicinal Chemistry, Temple, Jr. C., et al. "Preparation and Purification of L-(.+-.)-5-Formyl-5,6,7,8-tetrahydrofolic Acid," vol. 22, No. 6, p. 731 (1979).
Tetrahedron, Rees, L., et al. "Asymetric Reduction of Dihydrofolate Using Dihydrofolate Reductase and Chiral Boron-Containing Compounds," vol. 42, No. 1, p. 117 (1986).
Methods in Enzymology, Reyes, P., et al., "Dihydrofolate Reductase: A Coupled Radiometric Assay," vol. 122. p. 360 (1986).

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