Process for producing immunostimulants

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

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426 43, 435 681, 514885, 530322, 530825, A61K 3718, C07K 112, C12P 2106, A23C 2108

Patent

active

051853213

ABSTRACT:
Immunostimulant products are prepared by adding lysozyme to a culture of L.bulgaricus at a pH of from 4-8 and then incubating the culture to hydrolyze peptidoglycans of cell walls of the bacteria. A suspension containing the lysozyme-treated L.bulgaricus is collected, which is centrifuged to obtain an immunostimulant supernatant. The supernatant may be filtered to obtain an immunostimulant solution, which may be added to a fermented milk, or to a whey, to prepare an immunostimulant product. Alternatively, L.bulgaricus may be cultured and hydrolyzed by lysozyme in a milk product to provide an immunostimulant milk product.

REFERENCES:
patent: 3762998 (1973-10-01), Bogdanov
patent: 4501693 (1985-02-01), d'Hinterland et al.
patent: 4515891 (1985-05-01), Yokegawa et al.
Pahud et al, "Calf rennet lysozyme" Biochem. J., vol. 201, 1982, pp. 661-664.
Kuwana, et al. "Effect of Lytic Enzyme on Protoplast Formation of Lactic Acid Bacteria" Seikatsu Eisei (Life and Hygiene), vol. 31(2) 1987, pp. 32-41 (with translation).
Neviani, et al., "Sensitivity of Lactic Bacteria to Lysozyme" L'Industria del latte 24(1), 1988 (with translation).
Nomoto, et al. "Augmentation of resistance of mice to bacterial infection by a polysaccharide-peptidoglycan complex (PSPG) extracted from Lactobacillus casei", Biotherapy. 1:169-177, 1989.

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