Process for producing hydroxynitrile lyases

Details Process for producing hydroxynitrile lyases Process for producing hydroxynitrile lyases

2007-03-27

2007-03-27

Achutamurthy, Ponnathapu (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

C435S069100, C435S252330, C435S320100, C536S023200

Reexamination Certificate

active

10169430

ABSTRACT:
The present invention relates to an improved process for producing hydroxynitrile lyases. A process is provided whereby hydroxynitrile lyases may be produced by cultivation in bacteria or suitable host cells. Cells containing a gene encoding for a hydroxynitrile lyase may be precultivated, induced with a low concentration of IPTG, cultured, and lysed. The process allows for the production of relatively large quantities of hydroxynitrile lyases in a dissolved, native, and active form, and without the presence of substantial amounts of inclusion bodies, and thus allowing for the production of the lyases without requiring expensive and time consuming renaturation steps.

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Hasslacher et al., High-Level Intracellular Expression of Hydroxynitrile Lyase from the Tropical Rubber TreeHevea brasiliensisin Microbial Hosts, Protein Expression and Purification 11, (1997), pp. 61-71, Article No. PT970765, Academic Press.
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High-Level Intracellular Expresson of Hydroxynitrile Lyase from the Tropical Rubber TreeHevea brasiliensisin Microbial Hosts; Meinhard Hasslacher, et al; Protein Expression and Purification 11, 61-71 (1997) Article No. PT970765.
Molecular Cloning of the Full-length cDNA of (S)-Hydroxynitrile Lyase fromHevea brasiliensis; Meinhard Hasslacher, et al; vol. 271, No. 10, Issue of Mar. 8, pp. 5884-5891, 1996—The Journal of Biological Chemistry.

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