Process for producing human thrombin by gene modification...

Details Process for producing human thrombin by gene modification... Process for producing human thrombin by gene modification...

2002-07-04

2009-12-22

Robinson, Hope A (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S069100, C435S006120, C536S023100

Reexamination Certificate

active

07635577

ABSTRACT:
A genetic recombinant human thrombin is provided. Human thrombin is efficiently prepared by the genetic engineering technique comprising the steps: (1) culturing a transfectant animal cell transfected with an expression vector in which a gene encoding human prethrombin is incorporated to the downstream of a promoter so as to produce and accumulate prethrombin in culture supernatant and recovering the produced human prethrombin; (2) treating a solution containing human prethrombin recovered in step (1) with ecarin so as to convert human prethrombin into human thrombin; and (3) purifying the solution obtained after the above activation process to obtain purified human thrombin. The present invention allows for provision of human thrombin in a large scale in a safe and economical manner due to exclusion of blood-derived components.

REFERENCES:
patent: 5693499 (1997-12-01), Yonemura et al.
patent: 3168087 (1991-07-01), None
patent: 9204378 (1992-03-01), None
patent: 9600577 (1996-01-01), None
patent: WO 96/41868 (1996-12-01), None
patent: WO 99/58699 (1999-11-01), None
patent: WO 01/04146 (2001-01-01), None
Seop So, In et al “Purification and Activation of Recombinant Human Prethrombin 2 Produced inE. coli” Korean Biochem. J. (1992) vol. 25, No. 1, pp. 60-65.
Russo, et al., “Stable expression and purification of a secreted human recombinant prethrombin-2 and its activation to thrombin”, Protein Expression and Purification. 1997, vol. 10, No. 2, 214-225.
Nishida, et al., “cDNA cloning and deduced Amino acid sequence of prothrombin activator (ecarin) from Kenyan Echis carinatus venom”, Biochemistry. 1995, vol. 34, No. 5, 1771-1779.

Affiliated with

Also associated with

Rating

Process for producing human thrombin by gene modification... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for producing human thrombin by gene modification..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for producing human thrombin by gene modification... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4058732