Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1997-07-17
2000-01-04
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435212, 435219, 4352523, 43525233, 4353201, 536 231, 536 232, 536 235, C12N 964, C07H 2104
Patent
active
060108932
ABSTRACT:
The present invention relates to a process for producing a human matrilysin characterized in that a human promatrilysin is expressed in E. coli and secreted into the periplasm thereof; the inclusion body is formed, the human promatrilysin is solubilized with a urea solution, purified and renatured to obtain the active enzyme (active-type matrilysin). By this process, the active human matrilysin can be easily produced from transformed E. coli.
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Nashed Nashaat T.
Oriental Yeast Co. Ltd.
Wax Robert A.
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