Process for producing human interferon and method for assaying t

Details Process for producing human interferon and method for assaying t Process for producing human interferon and method for assaying t

1984-06-26

1988-05-17

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 29, 435 7, 435811, 436 63, 436 64, 436813, C12Q 170, C12Q 102, G01N 3353

Patent

active

047450533

ABSTRACT:
A process for producing HuIFN using whole human blood and a method for assaying the blood interferon (HuIFN) productivity are disclosed. The whole blood is incubated in the presence of an anticoagulant (e.g. heparin, ACD, and CPD) and a viral inducer under the conditions appropriate to accumulate a substantial amount of HuIFN. The blood HuIFN productivity determined by titrating the accumulated HuIFN with a suitable procedure (bioassay, radioimmunoassay, or enzyme-linked immunosorbent assay) is useful in clinical test to detect cancer in its early stage. The HuIFN per se is recovered, and purified prior to its prophylactic and therapeutic uses.

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