Process for producing highly purified HCG

Details Process for producing highly purified HCG Process for producing highly purified HCG

1986-04-21

1987-05-12

Phillips, Delbert R.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530416, 530418, 530422, 530424, C07K 312

Patent

active

046651616

ABSTRACT:
Crude HCG is purified by extracting with a neutral or weakly basic aqueous solution containing lower aliphatic alcohol and soluble salt, adding lower aliphatic alcohol to the extracted solution to form precipitates and the precipitates containing high purity of HCG are collected. This precipitates can be further purified by dissolving in a buffer solution, contacting the solution with a weak anion exchanger and eluting the exchanger with said buffer solution containing added salt.

REFERENCES:
patent: 4196123 (1980-04-01), Rosemberg
Van Hell, Chemical Abstracts, 83 (3), 1974, abst. No. 24700t.
Lauriere, Chemical Abstracts, 88 (23), 1977, abst. No. 166334d.
Tsubota et al., Chemical Abstracts, 89 (1), 1978, abst. No. 2739j.
Lee et al., Chemical Abstracts, 83 (25), 1974, abst. No. 203406y.
Okumura et al., Chemical Abstracts, 84, 1976, abst. No. 161407n.

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