Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-05-01
1999-08-17
Martinell, James
Chemistry: molecular biology and microbiology
Vector, per se
4352523, 43525231, 43525233, 435 691, 536 234, C12N 1500
Patent
active
059393157
ABSTRACT:
Processes for producing various heterologous polypeptides which when expressed are either incorrectly processed and hence asssociated with the surface of the host cell or are not processed to mature form. More specifically, processes for the production of heterologous non-human carbonyl hydrolases expressed either in host cells incapable of producing enzymatically active endoprotease or host cells deficient in enzymatically active extracellular endoprotease are disclosed. Such non-human carbonyl hydrolases generally are incapable of autoproteolytic maturation and become associated with the surface of expression hosts which are deficient in enzymatically active extracellular endoprotease. Processes for preparing non-human carbonyl hydrolase and heterologous polypeptides which are expressed as part of a fusion polypeptide are also disclosed, as well as non-human carbonyl hydrolases which are substantially free of the host cell membrane with which they are normally associated.
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Adams Robin Margaret
Power Scott Douglas
Powers David Brian
Wells James Allen
Yansura Daniel George
Martinell James
Silva, Esq. Robin M.
Trecartin, Esq. Richard F.
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