Process for producing heparinase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase

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435815, 435850, C12N 988

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active

043418694

ABSTRACT:
Heparinase is produced by growing the bacteria, Flavobacterium heparinum, in a defined medium consisting of a carbon source, two or more amino acids and mineral salts in the absence of protein. Heparinase is recovered by batch chromatography of the cell extract from hydroxylapatite by elution with sodium chloride and sodium phosphate buffer washes.

REFERENCES:
patent: 3549500 (1970-12-01), Suzuki
Applied and Environmental Microbiology, vol. 41, pp. 360-365, (Feb. 1981).
Linker et al. in Methods in Enzymology, vol. 28, pp. 902-911, (1972).
Hovingh et al. in The Journal of Biological Chemistry, vol. 245, No. 22, pp. 6170-6175, (Nov. 25, 1976).

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