Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase
Patent
1980-08-25
1982-07-27
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Lyase
435815, 435850, C12N 988
Patent
active
043418694
ABSTRACT:
Heparinase is produced by growing the bacteria, Flavobacterium heparinum, in a defined medium consisting of a carbon source, two or more amino acids and mineral salts in the absence of protein. Heparinase is recovered by batch chromatography of the cell extract from hydroxylapatite by elution with sodium chloride and sodium phosphate buffer washes.
REFERENCES:
patent: 3549500 (1970-12-01), Suzuki
Applied and Environmental Microbiology, vol. 41, pp. 360-365, (Feb. 1981).
Linker et al. in Methods in Enzymology, vol. 28, pp. 902-911, (1972).
Hovingh et al. in The Journal of Biological Chemistry, vol. 245, No. 22, pp. 6170-6175, (Nov. 25, 1976).
Cooney Charles L.
Galliher Parrish M.
Langer, Jr. Robert S.
Linhardt Robert
Cook Paul J.
Massachusetts Institute of Technology
Shapiro Lionel M.
Smith, Jr. Arthur A.
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