Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2005-07-12
2005-07-12
Lilling, Herbert J. (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
active
06916638
ABSTRACT:
Disclosed is a method for producing glycine, which comprises subjecting an aqueous solution of glycinonitrile to a hydrolysis reaction in a hydrolysis reaction system under the action of a microbial enzyme, thereby converting the glycinonitrile to glycine while by-producing ammonia, wherein the hydrolysis reaction system contains at least one organic impurity compound inhibiting the microbial enzyme, wherein the organic impurity compound has a molecular weight of 95 or more and contains at least one member selected from the group consisting of a nitrile group, a carboxyl group, an amide group, an amino group, a hydroxyl group and a trimethyleneamine structure, and wherein the hydrolysis reaction is performed under conditions wherein, during the hydrolysis reaction, the content of the organic impurity compound inhibiting the microbial enzyme in the hydrolysis reaction system is maintained at a level of 10% by weight or less, based on the weight of the hydrolysis reaction system.
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Ingvorsen et al., Ciba Foundation Symposium, vol. 140, pp. 16-31, (1988).
Aoki Toshiya
Kawakami Kiyoshi
Otsubo Kazumasa
Asahi Kasei Kabushiki Kaisha
Birch & Stewart Kolasch & Birch, LLP
Lilling Herbert J.
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