Process for producing glutathione

Details Process for producing glutathione Process for producing glutathione

2005-06-07

2005-06-07

Lilling, Herbert J. (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

C435S068100, C435S069100, C435S171000, C435S255200, C435S255210, C435S255400, C435S255500, C435S256800

Reexamination Certificate

active

06902912

ABSTRACT:
There is disclosed a fermentation process for producing glutathione which comprises (a) the obtainment of a biomass pre-culture by pre-cultivating, in aerobic conditions, a strain of a glutathione producing yeast wherein the glutathione content per biomass unit is higher than 1.2% w/w; (b) the cultivation, in aerobic conditions, of the resulting biomass pre-culture such that the resulting biomass density is higher than 50 g/l; (c) the activation of the cultured biomass; and (d) the recovery of the cultured biomass, extracting glutathione at a pH equal to or lower than 6 and purifying the resulting glutathione. The process allows to obtain glutathione with high yields and relatively low costs.

REFERENCES:
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patent: 0 146 265 (1985-06-01), None
patent: 2 692 280 (1993-12-01), None
C. Alfafara, et al., Applied Microbiology and Biotechnology, vol. 37, No. 2, XP-001032594, pp. 141-146, “Cysteine Addition Strategy for Maximum Glutathione Production in Fed-Batch Culture ofSaccharomyces cerevisiae”, 1992.
C. G. Alfafara, et al., Applied Microbiology and Biotechnology, vol. 36, No. 4, XP-008012042, pp. 538-540, “Effect of Amino Acids on Glutathione Production bySaccharomyces cerevisiae”, 1992.
G. R. Willsky, et al., Journal of Cellular Biochemistry-Supplement, No. 21A, XP-002179477, p. 251, “Vanadate Metabolism inSaccharomyces cerevisiae”, 1995.
H. Shimizu, et al., Biotechnology and Bioengineering, vol. 38, XP-002179478, pp. 196-205, “Optimal Production of Glutathione by Controlling the Specific Growth Rate of Yeast in Fed-Batch Culture”, 1991.
Derwent Publications, AN 1986-078827, XP-002179483, JP 61-027999, Feb. 7, 1986.
Derwent Publications, AN 1974-42553V, XP-002179481, JP 49-014680, Feb. 8, 1974.
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