Process for producing fructose 2,6,-bisphosphate and purificatio

Details Process for producing fructose 2,6,-bisphosphate and purificatio Process for producing fructose 2,6,-bisphosphate and purificatio

1995-11-27

1999-10-19

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 89, 435 92, 435101, 435105, 435194, C12P 1924, C12P 1902

Patent

active

059687878

ABSTRACT:
This invention relates to a process for the production of fructose 2,6-bisphosphate which comprises effecting reactions among (i) fructose 6-phosphate, (ii) glucose, (iii) fructose or (iv) glucose 6-phosphate, ATP and a phosphate donor, in the presence of (i) fructose 6-phosphate 2-kinase (PFK 2) and an enzyme which converts ADP into ATP (ADP/ATP converting enzyme), (ii) PFK 2, an ADP/ATP converting enzyme, hexokinase or glucokinase and glucose 6-phosphate isomerase, (iii) PFK 2, an ADP/ATP converting enzyme and hexokinase or glucokinase, or (iv) PFK 2, an ADP/ATP converting enzyme and glucose 6-phosphate isomerase; to a process for the production of fructose 2,6-bisphosphate which comprises allowing diesterase to coexist in a solution containing fructose 1,2-cyclic, 6-bisphosphate; and to a process for the purification of fructose 2,6-bisphosphate which comprises adding zinc salt to a solution containing fructose 2,6-bisphosphate, removing formed precipitate of impurities and adding a zinc salt to the resulting solution to isolate formed precipitate of fructose 2,6-bisphosphate. According to the present invention, fructose 2,6-bisphosphate can be produced easily with a high yield and high purity fructose 2,6-bisphosphate can be obtained easily.

REFERENCES:
patent: 5094947 (1992-03-01), Nakajima et al.
Pilkis et al "Chemical Synthesis of Fructose 2,6-Bisphosphate" Methods in Enzymology vol. 89 Carbohydrate Metabolism Part D (1982) pp. 101-107.

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