Process for producing foreign protein in Escherichia coli

Details Process for producing foreign protein in Escherichia coli Process for producing foreign protein in Escherichia coli

1991-02-08

1994-04-19

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352351, 4353201, 935 31, 935 38, C12P 2102, C12N 701, C12N 1570, C12N 1500

Patent

active

053044711

ABSTRACT:
A process of producing a foreign protein is provided which comprises transforming E. coli with a plasmid carrying a fused DNA having a DNA fragment encoding a foreign protein located downstream of a transcription initiation signal and translation initiation signal both derived from an appropriate T4 phage gene, infecting the transformant with a T4 phase denB and/or alc mutant at a low multiplicity of infection, and then culturing the infected transformant. The desired foreign protein can be produced in an extremely high yield, as compared to the prior art technique.

REFERENCES:
patent: 4845031 (1989-07-01), Shub et al.
Shub et al., "Bacteriophage T4, a New Vector for the Expression of Cloned Genes", Gene, 37:31-36, (1985).
Noguchi et al., Chemical Abstracts, vol. 105, 1986, Abstract No. 220261, (JP 61, 162, 168; Jul. 1986).
Duvoisin et al., Gene, vol. 45, (1986), pp. 193-201.
Snyder et al., Pro. Natl. Acad. Sci. U.S.A., vol. 73, No. 9, pp. 3098-3102.

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