Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1996-07-05
1998-07-07
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
53612313, C12P 1912, C07H 304
Patent
active
057767394
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to a process for producing disaccharides. The disaccharides are useful as a component of medicines, foods, and cosmetics, etc., and as an enzyme stabilizer in diagnostic reagents, etc.
BACKGROUND OF THE INVENTION
As a process for producing disaccharides having .alpha.,1-1 glucoside bond, there has been known a process for producing trehalose genus Nocardia (Japanese Published Unexamined Patent Application No. 154485/75) and a process for producing trehalose by treating maltose referred to as MP) and trehalose phosphorylase (hereinafter, referred to as TP) (Japanese Published Examined Patent Application No. 60998/88). As a process for producing disaccharides having .alpha.,1-4 glycoside bond, there has been known a process for producing maltose derivatives using an (1961)!.
However, in the process using the microorganism belonging to the genus Nocardia, only a slight amount of trehalose is produced in the culture medium, and in the process for producing disaccharides by the treatment of maltose with MP and TP, disaccharides other than trehalose cannnot be produced. In the process using an extract derived from Neisseria perflava, the substrate .beta.-glucose-1-phosphate is obtained by the phosphorolysis of maltose, however, the process requires a step of removing glucose formed as a by-product.
The disaccharides such as trehalose and maltose are known to enhance the Bio/Technology, 10, 1007-1011, (1992)!.
DISCLOSURE OF THE INVENTION
The present invention provides a process for producing disaccharides which comprises: conducting the condensation reaction of .beta.-glucose-1-phosphate with a monosaccharide in an aqueous medium in the presence of an enzyme source which is derived from a microorganism belonging to the genus Catellatospora, Kineosporia, Propionibacterium, or Enterococcus and which has sugar phosphorylase activity; and recovering the disaccharide formed in the aqueous medium. The present invention further provides disaccharides obtained by the process of the present invention.
The present invention is described in detail below.
The enzyme source employed in the present invention includes a culture, cells or processed cells of a microorganism having sugar phosphorylase activity or an enzyme having sugar phosphorylase activity.
As the microorganism having the sugar phosphorylase activity, any microorganism may be employed, so long as it belongs to the genus Catellatospora, Kineosporia, Propionibacterium, or Enterococcus and it is capable of producing an enzyme having the sugar phosphorylase activity. Examples of such microorganism include TP-producing microorganisms such as Catellatospora ferruginea KY2039 and Kineosporia aurantiaca ATCC 29727 and MP-producing microorganisms such as Propionibacterium freudenreichii KY4002 and Enterococcus faecium ATCC 10541.
The bacteriological properties of the species of the microorganisms listed above are described in Int. J. Syst. Bacteriol., 36, 512-517 (1986) for Catellatospora ferruginea, Bergey's Manual of Systematic Bacteriology, Vol.4, 2504-2506 (1989) for Kineosporia aurantiaca, Bergey's Manual of Systematic Bacteriology, Vol.2, 1346-1350 (1986) for Propionibacterium freudenreichii and Bergey's Manual of Systematic Bacteriology, Vol.2, 1063-1065 (1986) for Enterococcus faecium.
Catellatospora ferruginea KY2039 and Propionibacterium freudenreichii KY4002 were deposited with National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Japan on Jun. 11, 1993, under the Budapest Treaty, with the accession numbers FERM BP-4329 and FERM BP-4330, respectively.
As the medium employed to obtain the enzyme source having sugar phosphorylase activity, any of natural or synthetic medium may be employed, so long as it contains suitable amounts of carbon sources, nitrogen sources, minerals and other nutritients.
As the carbon source, carbohydrates such as glucose, sucrose, trehalose, maltose, starch and molasses, alcohols such as glycerol, sorbitol and mannitol, and organic acids
REFERENCES:
patent: 5565341 (1996-10-01), Takahashi et al.
Chemical Abstract of Japanese patent 08280395 A2 (Ajinimoto), Oct. 1996.
Colaco et al. "Extraordinary stability of enzymes dried in trehalose: Simplified Molecular Biology," Bio/Technology 10: 1007-1011, 1992.
Selinger, et al. "Enzymatic synthesis of the maltose analogues, glucosyl . . . ", J. Biol. Chem. 236: 2183-2185, 1961.
Aisaka Kazuo
Saitoh Yutaka
Uosaki Youichi
Ceperley Mary E.
Kyowa Hakko Kogyo Co. Ltd.
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