Process for producing cytidine diphosphate choline

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

Reexamination Certificate

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C435S189000, C435S194000, C435S193000, C435S183000, C435S440000, C435S252300, C435S320100, C435S252800, C435S069100, C435S071100, C435S004000, C435S006120, C536S023200, C536S023700, C536S023740, C536S023400

Reexamination Certificate

active

10113573

ABSTRACT:
A substantially pure culture of a microorganism having enzyme activities of pyrG and CCT and carrying a recombinant DNA composed of a DNA fragment containing genes encoding pryG and CCT and a vector.

REFERENCES:
patent: 4774180 (1988-09-01), Toth et al.
Tsukagoshi et al. Expression inEscherichia coliof the Saccharomyces cerevisiae CCT gene encoding cholinephosphate cytidylyltransferase,J Bacteriol. Mar. 1991;173(6):2134-6.
Hosaka et al. Cloning and characterization of the yeast CKI gene encoding choline kinase and its expression inEscherichia coli.J Biol Chem. Feb. 5, 1989;264(4):2053-9.
Weng et al. Nucleotide sequence ofEscherichia colipyrG encoding CTP synthetase. J Biol Chem. Apr. 25, 1986;261(12):5568-74.
Sambrook et al. Molecular cloning: a laboratory Manual. Cold Spring Harbor, 1989, 1.3-1.10 and 258.
Martin et al. Harper's Review of biochemistry, Lange Medical Publications, 18th edition, 1981, pp. 214-215 and 341.

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