Process for producing crystalline nucleus and method of...

Single-crystal – oriented-crystal – and epitaxy growth processes; – Processes of growth from liquid or supercritical state – Having growth from a solution comprising a solvent which is...

Reexamination Certificate

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C117S069000, C117S070000, C117S904000, C117S925000, C117S926000, C117S927000

Reexamination Certificate

active

10525809

ABSTRACT:
The present invention relates to a process for producing high-quality crystals of protein or organic substances easily and efficiently. A solution of protein or an organic substance is prepared and then is cooled slowly to be supersaturated to a low degree. This supersaturated solution is irradiated with a femtosecond laser10. A local explosion phenomenon occurs at the focal point of the laser and thereby a crystalline nucleus is generated. A high-quality crystal is obtained when a crystal is grown on the crystalline nucleus over a long period of time. The femtosecond laser to be used herein can be a titanium:sapphire laser having a wavelength of 800 nm, a duration of 120 fs, a frequency of 1 kHz, and an output of 400 mW.

REFERENCES:
patent: 7160820 (2007-01-01), Suga et al.
patent: 2005/0181464 (2005-08-01), Edwards et al.
patent: 2002-68899 (2002-03-01), None
patent: 2003-306497 (2003-10-01), None
Adachi, et al., “Laser Irradiated Growth of Protein Crystal”, Jpn. J. Appl. Phys., vol. 42 (2003), pp. L798-L800.
Bancel, et al., “Laser seeding for biomolecular crystallization”, Journal of Crystal Growth 191 (1998) 537-544.
Preuss, et al., “Time resolved dynamics of subpicosescond laser ablation ”, Appl. Phys. Lett. 62 (23), Jun. 7, 1993, pp. 3049-3051.
Garetz, et al., “Nonphotochemical, Polarization-Dependent, Laser-Induced Nucleation in Supersaturated Aqueous Urea Solutions”, vol. 77, No. 16, Oct. 14, 1996, pp. 3475-3476.

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