Process for producing carbohydrates using β...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S193000, C536S023200

Reexamination Certificate

active

07005279

ABSTRACT:
The present invention provides a novel polypeptide having a β1,3-N-acetylglucosaminyltransferase activity; a method for producing the polypeptide; a DNA which encodes the polypeptide; a recombinant vector into which the DNA is inserted; a transformant comprising the recombinant vector; a method for producing a sugar chain or complex carbohydrate, using the polypeptide; a method for producing a sugar chain or complex carbohydrate, using the transformant; an antibody which recognizes the polypeptide; a method for screening a substance which changes the expression of the gene which encodes the polypeptide; and a method for screening a substance which changes the activity of the polypeptide.

REFERENCES:
patent: 98/21328 (1998-05-01), None
patent: 98/44112 (1998-10-01), None
patent: 00/15826 (2000-03-01), None
patent: 01/85177 (2001-11-01), None
S. Di Virgillio et al. “Enzymatic synthesis of natural and C-13 enriched linear poly-N-acetyllactosamines as ligands for galectin-1”, Glycobiology 9(4): 353-364. (Apr. 1999).
D. Zhou et al. A Beta-1,3-N-acetylglucosaminyltransferase with poly-N-acetyllactosamine synthase activity is structurally related to Beta-1, 3-galactosyltransferases, PNAS 96: 406-411. (Jan. 1999).
K. Sasaki et al. “Expression cloning of cDNA encoding a human beta-I,3-N-acetylglucosaminyltransferase that is essential for poly-N-acetyllactosamine synthesis”, PNAS 94: 14294-14299. (Dec. 1997).
R. Almeida et al. “A Family of Human b-4-Galactosyltransferases. Cloning and Expression of Two Novel UDP-Galactose: b-N-Acetylglucosamine b-1,4-Galactosyltransferases, beta4Gal-T2 and beta4Gal-T3”, J. Biol. Chem 272(51): 31979-31991. (Dec. 1997.
T. Schwientek et al. “Cloning of a Novel Member of the UDP-Galactose: beta-N-Acetylglucosamine beta1,4-Galactosyltransferase Family, beta4Gal-T4, Involved in Glycosphingolipid Biosynthesis”, J. Biol. Chem 273(45): 29331-29340. (Nov. 1998).
Zhou, et al., “Homo sapiens beta-1,3-N-acetylglucosaminyltransferase mrRNA, complete cds”, GenBank Accession AF092051 (Jan. 1999).

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