Process for producing biologically active factors

Details Process for producing biologically active factors Process for producing biologically active factors

1983-04-05

1986-11-04

Rosen, Sam

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435681, C12P 2106

Patent

active

046210555

ABSTRACT:
A process for producing biologically active factors from a substrate, in the form of cell homogenates of organ tissues, of microorganisms, plant components and/or body fluids. To this end, the substrate, in an aqueous form and freed of accompanying particulate substances, is selectively separated by affinity chromatography using a biological sorbent, in the case of which at least one nucleic acid (desoxyribonucleic and/or ribonucleic acid) or at least one protein or peptide is coupled to a carrier substance; in the primary eluate components having no affinity are present, while the active factors (which have an affinity) are secondarily eluated. By binding nucleic acids or proteins from a given origin to a carrier, active factors with special properties such as tumor inhibiting substances, or stimulating substances may be positively produced.

REFERENCES:
Lowe et al.--Affinity Chromatography (Pub. Wiley & Sons) 1974, pp. 6, 7 & 129-133.

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