Process for producing ascorbic acid-2-phosphate

Details Process for producing ascorbic acid-2-phosphate Process for producing ascorbic acid-2-phosphate

1991-06-21

1993-10-05

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing organic compound containing a metal or atom other...

435132, 435194, 4353201, 43525233, 435874, 536 232, C07H 2104, C12N 912, C12N 1554, C12P 900

Patent

active

052504255

ABSTRACT:
Disclosed is a process for producing ascorbic acid-2-phosphate which comprises the steps of reaction, in aqueous medium, of ascorbic acid with a phosphate donor in the presence of cells, treated cells, a culture broth or a treated culture broth, of a microorganism belonging to the genus Escherichia and carrying a recombinant DNA comprising a gene isolated from a microorganism belonging to the species Pseudomonas azotocolligans and coding for ascorbic acid-phosphorylating enzyme until a recoverable amount of ascorbic acid-2-phosphate is accumulated in the aqueous medium, and recovery of ascorbic acid-2-phosphate therefrom.

REFERENCES:
Maruyama, A. et al., "Enzymatic Production of Ascorbic Acid-2-phosphate," Agric. Biol. Chem., 54(9), 2309-2313, (1990).

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