Process for producing amides by use of microoganisms

Details Process for producing amides by use of microoganisms Process for producing amides by use of microoganisms

1987-12-10

1990-03-13

Lipovsky, Joseph A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing nitrogen-containing organic compound

435191, C12P 1302, C12N 906

Patent

active

049083139

ABSTRACT:
In a process for hydrating a nitrile compound by the action of a microorganism having nitrilase activity to convert the nitrile compound into the corresponding amide compound, the performances such as yield and reaction velocity are markedly enhanced by irradiation of the microorganism with light. The process of the present invention is characterized in that (a) the microorganism having nitrilase activity is a positive gram-staining microorganism, (b) the microbial cells are allowed to accept light energy of at least about 1.times.10.sup.-2 .mu.E/g microbial cells second before termination of the hydration reaction, and (c) the hydration reaction is carried out in a vessel composed at least partly of a non-light transmitting material.

REFERENCES:
patent: 3880717 (1975-04-01), Rubin et al.
Hug et al., Journal of Bacteriology, 102, 874-876 (1970).
Hug et al., Photochemistry and Photobiology, 13, 171-177 (1971).
Hug et al., Biochemistry, 10, 1397-1401 (1971).
Hug et al., Journal of Biological Chemistry, 253, 7622-7629 (1978).

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