Process for producing a targeted gene

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435238, 435239, 435370, C12Q 170, C12N 706, C12N 702, C12N 508

Patent

active

056838662

ABSTRACT:
This invention relates to a reconstituted sendai-viral envelope containing the F-protein (F-virosomes) and to a process for producing a targeted gene or drug delivery carrier produced by the steps of chemical reduction of Sendai virus for reduction of HN protein and subjecting the reduced virus to the step of dialysis for removal of the reducing agent. The reduced virus is then solubilized with a detergent to obtain a solution. The said solution is centrifuged to separate the insolubles consisting of reduced HN protein and core of the virus, adding the required specific gene or drug to the centrifugal solution. Finally, the detergent is removed using an affinity complex agent which binds the detergent leading to the formation of the delivery carrier.

REFERENCES:
Bagai and Sarkar. Fusion-mediated microinjection of lysozyme into HepG2 cells through hemmaglutinin neuramidase-depleted sendai virus envelopes. J. Biol. Chem.. vol. 269(3):1966-1972. Dec. 21, 1994.
Tomita et. al.. Direct in-vivo gene introduction into rat kidney. BBRC. vol. 186(1):129-134. Jul. 15 1992.

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