Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1988-06-10
1991-02-12
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 1, 436804, 436808, C12Q 116
Patent
active
049923668
DESCRIPTION:
BRIEF SUMMARY
The invention relates to the field of biochemical analytical methods and relates to a process for producing a stable receptor preparation for a stable (multicomponent) radioreceptor assay and to the radioreceptor assay obtained by this process, its kit and the use of said kit or said assay.
BACKGROUND OF THE INVENTION
In the same way as with a radioimmunoassay, the principle of the radioreceptor assay is based on the biospecific detection of a ligand (used in a planned manner for the assay), e.g. a hormone, pharmacon, neurotransmitter, etc. at the corresponding target or acceptor molecule, e.g. an antibody in the radioimmunoassay or a receptor in the radioreceptor assay. As a result of the radioactive labelling of such a ligand used in a planned manner, an observable molecule is obtained, i.e. the tracer, which is in competitive interaction with an unlabelled ligand and therefore the similar, but unobservable, molecule. Through such measures a test system is obtained, which makes it possible to measure an unknown concentration of such a ligand.
While radioimmunoassay has become a widely used routine method and is used to a significant extent as an analytical method at present, radioreceptor assay is only rarely used for this purpose.
One of the main reasons for this is that the handling of biologically active receptors is more difficult than with an antibody. The prerequisite for a routine method is, apart from relatively simple handling (method simplicity) and the accompanying economical aspects, mainly the "stability" of the chemical or biological reactant. This means the stability of the actual analysis substance and its stability within the analytical reaction.
However, in solution, biologically active receptors are unstable and must therefore be frozen solid until just before they undergo analytical use. In addition, they are so unstable that even if this condition is briefly not fulfilled, the receptor can be made unuseable. It is obvious that such sensitive characteristics make it impossible to use as a routine method such a fundamentally useable analysis mechanism.
SUMMARY OF THE INVENTION
The object of the invention is therefore to provide a way of supplying such sensitive substances to a simple routine method, which can be easily performed while being able to avoid the hitherto necessary measures such as freezing and the like with all the problems, disadvantages and risks associated therewith.
This objective is achieved through the preparation process for a radioreceptor assay and a radioreceptor assay produced by this process for direct analytical use.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT
The aim is that all or a maximum number of reactants can be present together in a common vessel and that the starting up of the bonding or binding reaction is performed with a single pipetting step, whereby apart from the material to be analysed, all the reaction participants are available in stable form, e.g. in a test tube. Conventional processes of this type generally require 3 to 4 pipetting stages, so that a considerable economic advance is provided in connection with such analytical methods made possible by the invention in a single process, (in connection with the speed of performing the test, the accuracy and the working expenditure). Moreover the invention leads to a test kit which, without quality loss, can be stored at ambient or refrigerator temperature (no freezing), which is particularly important in connection with transportation, because there is no need for the hitherto necessary dry ice for transportation purposes.
The process described hereinafter for the production of a stable radioreceptor assay leads to obtaining a dried, stable form.
The preparation of receptor-containing cell membranes is performed in accordance with methods described in the literature, e.g. according to E. Burgisser et al, Biochem. Biophys. Res. Commun., Vol.133, pp 1202-1209, 1985. This involves the obtaining of suitable tissue material or cells, e.g. blood cells or cell cultures. This is followed
REFERENCES:
patent: 4162003 (1979-07-01), Bartos et al.
patent: 4197288 (1980-04-01), Snyder
patent: 4259207 (1981-03-01), Fruitstone et al.
patent: 4461829 (1984-07-01), Greenquist
Buregisser et al., Biochem., Biophys. Res. Comm., vol. 133, pp. 1202-1209, 1985.
Anawa Laboratorien AG
Farley Walter C.
Stucker Jeffrey
Wax Robert A.
LandOfFree
Process for producing a receptor preparation for a radioreceptor does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for producing a receptor preparation for a radioreceptor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for producing a receptor preparation for a radioreceptor will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-20161