Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-05-07
1998-12-22
Weber, Jon P.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435383, C12P 2106, C12N 502
Patent
active
058518002
ABSTRACT:
A process is presented for reducing the detrimental influence of certain proteases on recombinant human protein and polypeptide production in a cell culture, which comprises adding an inhibitor of metal-dependent proteases or chymotrypsins to the cell culture medium. The cell culture medium for cultivating cells expressing and secreting a biologically active recombinant human polypeptide contains an inhibitor of metal-dependent proteases or chymotrypsins, or a combination thereof. Recombinant factor VIII which has been produced in a cell culture medium according to the present process is useful for the manufacture of a medicament for administration to a patient having the symptoms of hemophilia A and for treatment of hemophilia A by administration of a therapeutically effective amount of recombinant factor VIII.
REFERENCES:
patent: 4891356 (1990-01-01), Szabo
patent: 5149787 (1992-09-01), Kunicki et al.
patent: 5189178 (1993-02-01), Galardy et al.
patent: 5279939 (1994-01-01), Berka et al.
patent: 5296471 (1994-03-01), Holme et al.
patent: 5304603 (1994-04-01), Chemg et al.
patent: 5514578 (1996-05-01), Hogness et al.
patent: 5514585 (1996-05-01), Bjorn et al.
patent: 5543396 (1996-08-01), Powers et al.
patent: 5589373 (1996-12-01), Weiner et al.
patent: 5631159 (1997-05-01), Marshall et al.
patent: 5648237 (1997-07-01), Carter et al.
patent: 5661034 (1997-08-01), Hayakawa et al.
M. Tokunaga et al, Yeast Secretion of Mouse a-Amylase from Fission Yeast Senizosaccharomyces pombe: Presence of Chymostatin-sensitive Protease Activity in the Cultate Medium , vol. 9, pp. 379-387 (1993).
Ikegawa et al, Biochemical and Biophysical Research Communications, Phosphoramidon, A metalloprotemase Inhibitor, Suppresses the Secretion of Endothelin-1 from Cultured Endothelial Cells by Inhibiting a Big Endothelin-1 Converting Enzyme, vol. 171, No. 2, pp. 669-675 (1990).
Mitsuo Satoh et al, In Vitro Cell Dev. Biol, Chinese Hamster Ovary Cells Continuously Secrete A Cysteine Endopeptidase, vol. 26, pp. 1101-1104 (Nov. 1990).
J. S. Boud et al, Int. J. Biochem., Mammalian Metalloendopeptidases, vol. 17, No. 5, pp. 565-574 (1985).
Andersoon et al, Proc. Natl. Acad. Sci. Isolation and Characterization of Human Factor VIII: Molecular Forms in Commercial Factor VIII Concentrate, Cryoprecipitate, and Plasma, vol. 83, pp. 2979-2983 May 1986).
A. Barrett, Methods In Enzymology Proteolytic Enzymes: Asparatic and Metallo Peptidases: vol. 248, Academic Press, Inc., San Diego, CA, p. 263-283 (1996).
R. Kratje et al, Journal of Biotechnology, Evaluation of the Proteolytic Potential of in vitro-cultivated hybridoma and recombinant mammalian cells, vol. 32, pp. 107-125 (1994).
Stryer, Biochemistry Zymogen Activation: Digestive Enzymes and Clotting Factors, Chapter 8, WH Freeman and Co., San Francisco, CA, pp. 157-166 (1981).
N. Nishino et al, Biochemistry, Peptide Hydroxamic Acids as Inhibitors of Thermolysin, vol. 17, No. 14, pp. 2846-2850 (1978).
Adamson Lars
Dixelius Johan
Lie Kristina Lima
Walum Erik
Pharmacia & UpJohn AB
Weber Jon P.
LandOfFree
Process for producing a protein does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for producing a protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for producing a protein will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2046026