Process for producing a pharmaceutical preparation of PDGF-AB

Details Process for producing a pharmaceutical preparation of PDGF-AB Process for producing a pharmaceutical preparation of PDGF-AB

1997-01-02

1999-08-10

Ulm, John

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435325, 435360, 4353201, 530399, 514 2, 514 12, C12N 1516, C12N 1506, C12N 1563, A61K 3818

Patent

active

059358190

ABSTRACT:
The recombinant production of PDGF-AB in mammalian cells is disclosed by means of a bicistronic vector system in which an IRES sequence is located between the first and second cistrons and in which the B chain coding gene is located in the first cistron. The disclosed expression unit allows the equilmolar expression of the A and B polypeptide chains.

REFERENCES:
patent: 5035887 (1991-07-01), Antoniades et al.
patent: 5149691 (1992-09-01), Rutherford
patent: 5165938 (1992-11-01), Knighton
patent: 5334532 (1994-08-01), Tackney et al.
patent: 5358856 (1994-10-01), Baltimore et al.
patent: 5428135 (1995-06-01), Lyons et al.
Ostman et al., J. Biol. Chem. 263, pp. 16202-16208, 1988.
Eichner et al., Eur J. Biochem. 185, pp. 135-140, 1989.
Jackson et al., TIBS, 15:477-483, 1990.
Schneppe et al., Gene 143, pp. 201-209, 1994.
Hannink et al., "Deletions in the C-Terminal Coding Region of the v-sis Gene: Dimerization IS Required for Transformation", Molecular andCellular Biology 6(4):1304-1314 (1986).
King et al., "In vitro mutagenesis of the v-sis transforming gene defines functional domains of its growth factor-rel product", Proc. Natl. Acad. Sci. USA 82:5295-5299 (1985).
Sauer et al., "Deletions in the N-Terminal Coding Region of the v-sis Gene: Determination of the Minimal Transfo Region", Journal of Virology 59(2):292-300 (1986).
Haller et al., "Linker Scanning Mutagenesis of the Internal Ribosome Entry Site of Poliovirus RNA", Journal of V 66(8):5075-5086 (1992).
Betsholtz et al., "cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chai its expression in tumour cell lines", Nature 320(6064):695-699 (1986).

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