Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1997-07-15
1999-09-07
Tsang, Cecilia J.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
530322, 530345, 530402, 514 8, A61K 3814
Patent
active
059488956
ABSTRACT:
A process which makes possible to produce a glycosylated amino compound (e.g. protein) easily in short period without denaturation of protein. The sugar (e.g. glucose) and protein (e.g. serum) are mixed, and the mixture is dried under reduced pressure, so that sugar and protein quickly react with each other to produce the glycosylated protein. The drying under reduced pressure is preferably lyophilization which is conducted under the following conditions: the temperature is about -20.degree. C., the pressure is about 2 mmHg, and the treatment time is 12 hours. Unreacted glucose is then removed by the dialysis process, thus providing the required glycosylated protein sterilization need not be carried out. This process makes it possible to produce a glycosylated protein in about one day as compared to 7 days by the conventional process, so that the denaturation of protein is less likely to occur.
REFERENCES:
patent: 3919317 (1975-11-01), Huff
patent: 4508921 (1985-04-01), Amato
patent: 5032405 (1991-07-01), Huang
patent: 5122367 (1992-06-01), Ron
patent: 5583140 (1996-12-01), Beucherif
patent: 5614487 (1997-03-01), Battersby
patent: 5656721 (1997-08-01), Albert
Abstract of: Mora, M.S. et al., Developments in Biological Standardization vol. 74, 295-303, 1992.
E. Schleicher et al., "Specific Quantitation by HPLC of Protein (Lysine) Bound Glucose in Human Serum Albumin and Other Glycosylated Proteins", J. Clin. Chem. Clin. Biochem., vol. 19, pp. 81-87.
K. A. Mereish et al., "Glucosylated Albumin and its Influence on Salicylate Binding", Journal of Pharmaceutical Sciences, vol. 71, No. 2, pp. 235-238.
Marc Rendell et al., "Aminophenylboronic Acid Affinity Chromatography and Thiobarbituric Acid Colorimetry Compared for Measuring Glycated Albumin", Clincal Chemistry, vol. 31, No. 2, pp. 229-234.
Marc Rendell et al., "Use of aminophenylboronic acid affinity chromatography to measure glycosylated albumin levels", J Lab Clin Med, vol. 105, No. 1, pp. 63-69.
Yasuo Ohe et al., "Radioimmunoassay of glycosylated albumin with monoclonal antibody to glucitol-lysine", Clinica Chimica Acta, pp. 229-238.
Jencks, Catalysis in Chem & Enz, pp. 182-193, 1969.
Kunz, Angew Chem Int Ed. 26, 294, 1987.
Ohnaka Akane
Sugiyama Koji
Kyoto Dai-ichi Kagaku Co. Ltd.
Lukton David
Tsang Cecilia J.
LandOfFree
Process for producing a glycosylated amino compound does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for producing a glycosylated amino compound, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for producing a glycosylated amino compound will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1805505