Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1982-12-01
1986-04-08
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435253, 435317, 435189, 935 27, 935 60, C12N 1500, C12N 902, C12N 120, C12N 100
Patent
active
045813353
ABSTRACT:
A process for producing a cloned luciferase-synthesizing microorganism, capable of the expression of bioluminescence, is disclosed. The process involves isolation and digestion of the genomic DNA of an appropriate bioluminescent microorganism, such as Vibrio harveyi, to obtain a DNA fragment which codes for luciferase. The DNA fragment is inserted into a pre-existing cloning vehicle under the control of an expression promoter, thereby producing a recombinant or derivatized cloning vehicle. A host microorganism is transformed with the derivatized cloning vehicle, cultured and purified for the expression of bioluminescence.
REFERENCES:
Stuber et al., PNAS USA, vol. 78, pp. 167-171, Jan. 1981.
Belos et al., Science, vol. 218, pp. 791-793, Nov. 19, 1982.
Cohn et al., PNAS USA, vol. 80, pp. 120-124, Jan. 1983.
Lamfrom et al., Journal of Bacteriology, vol. 133, No. 1, pp. 354-363, (Jan. 1978).
Abstract of 82nd Annual Meeting of The American Society for Microbiology (ASMAC), #H123, Mar. 7-12, 1982.
Tanenholtz Alvin E.
Texas A&M University System
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