Process for producing 7B-(4-carboxybutanamido)-cephalosporanic a

Details Process for producing 7B-(4-carboxybutanamido)-cephalosporanic a Process for producing 7B-(4-carboxybutanamido)-cephalosporanic a

1996-09-11

1998-09-01

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4352523, 4353201, C12N 121, C12N 1570

Patent

active

058010483

ABSTRACT:
The present invention relates to a process for producing large amounts of 7.beta.-(4-carboxybutanamido)-cephalosporanic acid acylase ("GA") enzyme, which process consists in growing, under suitable culture conditions, a recombinant Escherichia coli K12 strain and subsequently extracting the enzyme from the resulting culture.
Enzyme production is controlled by a particular expression system which can be induced by corn-steep liquor, an extremely low cost component of the culture medium.
The enzyme can be used in the enzymatic preparation of 7-amino-cephalosporanic acid by starting from 7.beta.-(4-carboxybutanamido)-cephalosporanic acid.

REFERENCES:
Matsuda et al. "Cloning and characterization of the genes for two distinct ephalosporin acylases from a Pseudomonas strain" J. Bacteriology 169 (12): 5815-5812, Dec. 1987.
Brosius et al. "Regulation of ribosomal RNA promoters with a synthetic lac operator." Proc. Nat. Acad. Sci., USA 81: 6929-6933, Nov. 1984.
Matsuda et al. "Molecular cloning and structure of the gene for 7.beta.-(4-carboxybutanamido)cephalosporanic acid acylase from a Pseudomonas strain". J. Bacteriology 163 (3): 1222-1228, Jul. 1985.

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