Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1994-12-30
1997-03-18
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4351723, 4353201, 43525233, 435230, C12N 121, C12N 1555, C12N 980, C12N 1572
Patent
active
056122101
ABSTRACT:
The present invention relates to a process for producing large amounts of 7.beta.-(4-carboxybutanamido)-cephalosporanic acid acylase ("GA") enzyme, which process consists in growing, under suitable culture conditions, a recombinant Escherichia coli K12 strain and subsequently extracting the enzyme from the resulting culture.
Enzyme production is controlled by a particular expression system which can be induced by corn-steep liquor, an extremely low cost component of the culture medium.
The enzyme can be used in the enzymatic preparation of 7-amino-cephalosporanic acid by starting from 7.beta.-(4-carboxybutanamido)-cephalosporanic acid.
REFERENCES:
patent: 4359528 (1982-11-01), Devos et al.
patent: 4440792 (1984-04-01), Bradford et al.
Brosius et al. (1984) Proc. Natl. Acad. Sci., USA 81:6929-6933.
Agric. Biol. Chem., vol. 45, No. 10, pp. 2231-2236, 1981, Shigeaki Ichika et al., "Purification and Properties of 7.beta.-(4-Carboxybutanamido)-cephalosporanic Acid Acylase Produced By Mutants Derived From Pseudomonas".
Journal of Bacteriology, vol. 163, No. 3, pp. 1222-1228, Sep., 1985, Akio Matsuda, et al., "Molecular Cloning and Structure of the Gene for 7.beta.-(4-Carboxybutanamido)cephalosporanic Acid Acylase From a Pseudomonas Strain".
Journal of Bacteriology, vol. 169, No. 12, pp. 5815-5820, Dec. 1987, Akio Matsuda et al., "Cloning and Characterization of the Genes For Two Distinct Cephalosporin Acylases From A Pseudomonas Strain".
Journal of Bacteriology, vol. 173, No. 24, pp. 7848-7855, Dec. 1991, Ichiro Aramori, et al., "Cloning and Nucleotide Sequencing of a Novel 7.beta.-(-Carboxybutanamido)cephalosporanic Acid Acylase Gene of Bacillus Laterosporus and Its Expression In Escherichia coli and Bacillus subtilis".
Biochimica et Biophysica Acta, vol. 1132, pp. 233-239, 1992, Masayuki Ishiye, et al., "Nucleotide Sequence and Expression in Escherichia coli of the Cephalosporin Acylase Gene of a Pseudomanas Strain".
Agric. Biol. Chem., vol. 45, No.7, pp. 1561-1567, 1981, Yuzo Shibuya, et al., "Isolation and Properties of 7.beta.-(4-Carboxybutanamido)cephalosporanic Acid Acylase-Producing Bacteria".
Indian Journal of Experimental Biology, vol. 26, pp. 22-24, Jan. 1988, Malathi Srinivasan, et al., "Effect of Carbon and Nitrogen on Exoprotease Synthesis in Batch Cultures of Bacillus licheniformis NCIM 2042".
Bernardi Antonella
Bosetti Aldo
Cesti Pietro
Franzosi Giuliana
Van Der Goes Wilhelmus
Bugaisky Gabriele E.
Ministero Dell'Universita' e Della Ricerca Scientifica e Tecnolo
Wax Robert A.
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