Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-09-09
1996-03-05
Draper, Garnette D.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 698, 435 712, 4353201, 530399, C12P 2106, C12N 1524, A61K 3827, C07K 1461
Patent
active
054967139
ABSTRACT:
A human growth hormone having a molecular weight of 20,000 daltons can be effectively secreted and produced in the periplasm of Escherichia coli by constructing a human growth hormone secretion plasmid comprising a vector DNA replicable in E. coli and a DNA fragment including a promoter, a ribosome binding site, a secretion signal coding region, which are all those of the neutral protease gene of B. amyloliquefaciens and a gene encoding 20 kD hGH placed just downstream from the secretion signal coding region; introducing the said plasmid into E. coli; and culturing the resultant transformed cells.
REFERENCES:
Honjo et al. 1986 J Biotechnology 4:63-71.
Nakayama et al. 1987 J Biotechnology 6:171-179.
Honjo et al 1987 J Biotechnology 6:191-204.
Sambrook et al 1989 Molecular Cloning A Lab. Manual. CSHL Press, CSH, NY. Excepts Chapter 17 & Appendix.
Journal of Biotechnology, vol. 8, 1988, pp. 123-134, Makayama.
Gene, vol. 55, 1987, pp. 189-196.
Rogers et al, The Lancet, Aug. 27, 1977, pp. 434-435.
Fisher et al, The Lancet, May 21, 1988, pp. 1159-1160.
Horumon to Rinsho, vol. 38, 1990, pp. 155-159 (and translation of pertinent portions).
Endocrinol, Japon, vol. 36, 1989, pp. 9-13.
Endocrinol, Japon. vol. 34, 1987, pp. 73-85, Lewis.
Int'l. Symposium on GRF, Growth Hormone & Somatomedin Program & abstracts, 28, Nov. 1-2, 1986.
Gene, vol. 39, 1985, pp. 247-254.
J. Cell Biology, vol. 67, 1975, pp. 835-851.
Biochimica et Biophysica Acta, vol. 949 (1988), pp. 125-131.
Science, vol. 205, Aug. 10, 1979, pp. 602-607.
Trends in Biotech., vol. 10, 1992, pp. 310-315.
J. Biol. Chem., vol. 241, No. 13, Jul. 10, 1966, pp. 3055-3062.
Nucleic Acids Research, vol. 5, No. 7, Jul. 1978, pp. 2321-2332.
Journal of Immunology, vol. 116, No. 6, Jun. 1976, pp. 1554-1560.
Journal of Biol. Chemistry, vol. 255, No. 1, Jan. 1980, pp. 27-29.
Journal of Biotechnology, vol. 8, 1988, pp. 123-134.
Honjo Masaru
Naito Naokazu
Nakayama Akira
Yoshino Setsuo
Carlson K. Cochrane
Draper Garnette D.
Mitsui Toatsu Chemicals Inc.
LandOfFree
Process for producing 20 kD human growth hormone does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for producing 20 kD human growth hormone, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for producing 20 kD human growth hormone will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1412395