Process for preparing virus-resistant transgenic plant

Details Process for preparing virus-resistant transgenic plant Process for preparing virus-resistant transgenic plant

1994-10-06

1997-08-12

Fox, David T.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 4353201, 4352523, 800205, 800DIG42, 536 236, A01H 104, C12N 1500, C12N 514

Patent

active

056564660

ABSTRACT:
The present invention provides a process for preparing a transgenic plant producing antiviral protein, which is transformed with a recombinant vector for Phytolacca insularis antiviral protein(PIP) expression. In accordance with the present invention, a recombinant expression vector for Phytolacca insularis antiviral protein made a grant of broad vital-resistance to transgenic plant transformed therewith, grounded on the expression of PIP.

REFERENCES:
Owens, R. A. et al., A Possible Mechanism for the Inhibition of Plant Viruses by a Peptide from Phytolaca americana, Virology, 56:390-393(1973).
Irvin, J. D. et al., Pruification and Partial Charaterization of the Antiviral Protein from Phytolacca americana Which Inhibits Eukaryotic Protein Synthesis, Arch. Biochem. Biophys., 169:522-528(1975).
Irvin, J. D. et al., Purification and Properties of a Second Antiviral Protein from Phytolacca americana Which Inactivates Eukaryotic Ribosomes, Arch. Boichem. Biophys., 200:418-425 (1980).
Lin, Q. et al., Isolation and Characterization of a cDNA Clone Encoding the Antiviral Protein from Phytolacca americana, Plant Mol. Biol., 17:609-614(1991).
Ready, M. P. et al., Extracellular Localization of Pokeweed Antiviral Protein, Proc. Natl. Acad., USA, 83:5053-5056(1986).
Nejidat, A. et al., Engineered Resistance against Plant Virus Diseases, Physiol. Plant., 80:662-668(1990).
Cuozzo M. et al., Viral Protection in Transgenic Tobacco Plants Expressing the Cucumber Mosaic Virus Coat Protein or Its Antisense RNA, bio/Technology, 6:549-557(1988).
Lodge et al (Aug. 1993) Proc. Natl Acad. Sci USA 90:7089-7093.

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