Process for preparing uridine diphosphate-N-acetylgalactosamine

Details Process for preparing uridine diphosphate-N-acetylgalactosamine Process for preparing uridine diphosphate-N-acetylgalactosamine

1985-02-25

1986-08-05

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

424 11, C12Q 160

Patent

active

046043497

ABSTRACT:
A process for preparing uridine diphosphate-N-acetylgalactosamine, which comprises treating a reaction solution obtained by the enzymatic conversion of uridine diphosphate-N-acetylglucosamine to uridine diphosphate-N-acetylgalactosamine, with uridine diphosphate-N-acetylglucosamine pyrophosphorylase to decompose the remaining uridine diphosphate-N-acetylglucosamine in the solution and then separating therefrom the uridine diphosphate-N-acetylgalactosamine for purification. In one aspect of this invention, it relates to a method for measuring the activity of .alpha.-N-acetylgalactosaminyl transferase characterized by the use of said reaction solution as the substrate for the transferase.

REFERENCES:
Agricultural and Biological Chemistry, vol. 37(7), 1741-1743, (1973).
Methods in Enzymology, vol. 28, 1972, pp. 511-514; 655-657.

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