Process for preparing the principal proteins of hemolyzed blood

Details Process for preparing the principal proteins of hemolyzed blood Process for preparing the principal proteins of hemolyzed blood

1986-06-13

1988-08-16

Fisher, Richard V.

Liquid purification or separation

Processes

Chromatography

210730, 210743, 436 63, 436 66, 436175, B01D 1508, B01D 2100

Patent

active

047642793

ABSTRACT:
The process comprises subjecting the hemolyzed blood to a clarification step at a pH of between 4 and 6 in the presence of less than 15% of alcohol, then, after concentration, effecting a chromatography by exchange of anions so as to separate the hemoglobin, the albumin being thereafter eluted. The hemoglobin and the globulins may then be separated out by precipitation with 25% alcohol at a pH of 7. The proteins obtained are in the native state.

REFERENCES:
patent: 3637489 (1972-01-01), Haller
patent: 3657116 (1972-04-01), Haller
patent: 4297274 (1981-10-01), Bohn et al.
"Industrial Ion Exchange Chromatography of Proteins on DEAE Dextran Derivatives of Porous Silica Beds", Tayot et al. Chromatogr. Synth. Biol. Polym., (1978), 2, pp. 95-110.

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