Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Reexamination Certificate
2002-12-31
2004-03-16
Solola, Taofiq (Department: 1626)
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
Reexamination Certificate
active
06706901
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to an improved process for the preparation of taxol side chain that comprises the following steps: (a) Synthesis of (2R,3S)-2,3-dihydroxy-3-phenylpropionate with greater than 99% enantioselectivity and devoid of osmium even in the crude in a single pot using recyclable multifunctional catalysts of the formula IE-IE-PdOsW, in which IE is a ion-exchanger comprising LDH, quaternary ammonium salt anchored on silica, clay, alumina, magnesia or resin (b) conversion of the diol thus obtained without further crystallization into bromoacetate using standard protocol, (c) reaction of bromoacetate with NaN
3
in DMF followed by deacetylation with NaOAc in MeOH affords azido alcohol (d) benzoylation followed by hydrogenation of azido alcohol gave the (2R,3S)-(N-)-benzoyl-3-phenylisoserine methyl ester in 67% overall yield.
BACKGROUND OF THE INVENTION
Taxol isolated from the bark of several yew species, is considered to be the most promising cancer chemotherapeutic agent and has been approved for the treatment of metastatic carcinoma of the ovary. Central to all synthetic strategies for taxol is the attachment of the C-13 side chain to the baccatin III nucleus. Since the presence of this side chain has proved to be essential for the biological activity of taxol, the development of short and practical synthetic routes for phenylisoserine derivatives, which are adaptable for industrial-scale production has become very important.
Sharpless et al. developed a process for the taxol side chain through AD of methyl cinnamate that led to 23% overall yield, but the diol needs to be recrystallized to enrich the ee. As the trifunctional catalyst in the present case afforded the diol with 99% ee using the H
2
O
2
as a terminal oxidant, we present an efficient synthesis of taxol side chain starting from bromobenzene and methyl acrylate as shown in the Scheme 1. The treatment of diol(Methyl 2,3-dihydroxy-3-pheylpropionate) with HBr—AcOH yields broinoacelate, which in turn reacts with NaN
3
in DMF followed by deacetylation with NaOAc in MeOH to afford azido alcohol. Benzoylation followed by hydrogenation of azido alcohol gave the (2R,3S)-(N-)-benzoyl-3-phenylisoserine methyl ester in 67% overall yield. Reference is made to
J. Org. Chem
1994, 59, 5104 wherein optically active taxol C-13 side chain was prepared using chiral auxiliary followed by bromo acetoxylation. The inherent disadvantage is use of homogeneous catalyst and low overall yield. Reference is also made to
Ind. J. Chem
1995, 34B, 471 wherein optically active taxol C-13 side chain was prepared by asymmetric dihydroxylation using chiral auxiliary. The inherent disadvantage is recovery of entire amount of toxic osmium tetroxide used. Reference is made to
J. Org. Chem
1990, 55, 1957 wherein optically active taxol C-13 side chain was prepared by asymmetric dihydroxylation using chiral auxiliary. The inherent disadvantage is recovery of entire amount of toxic osmium tetroxide used.
OBJECTS OF THE INVENTION
The main object of the present invention relates to an improved process for the preparation of taxol side chain by synthesizing (2R,3S)-2,3-dihydroxy-3-phenylpropionate with greater than 99% enantioselectivity and devoid of osmium even in crude form in a single pot using a recyclable multifunctional catalysts, conversion of diol obtained without further crystallization into bromoacetate, reaction of bromoacetate with NaN
3
in organic solvent followed by deacetylation with to obtain azido alcohol, benzoylation followed by hydrogenation of azido alcohol to obtain the (2R,3S)-(N-)-benzoyl-3-phenylisoserine methyl ester in 67% overall yield.
Another object of the invention to provide a novel and ecofriendly process for synthesis of (2R,3S)-2,3-dihydroxy-3-phenylpropionate from bromobenzene and ethylacrylate in a single pot.
It is another object of the invention to provide a process for the synthesis of (2R,3S)-2,3-dihydroxy-3-phenylpropionate dispensing with the use of soluble and toxic osmium tetroxide or potassium osmate dihydrate.
It is a further object of the invention to provide a process for the synthesis of (2R,3S)-2,3-dihydroxy-3-phenylpropionate wherein both the enantioselectivity and yields obtained are higher than reported in homogeneous dihydroxylation.
It is another object of the invention to provide a process for the synthesis of (2R,3S)-2,3-dihydroxy-3-phenylpropionate wherein the work-up procedure is simple and economical.
It is yet another object of the invention to provide a process for the synthesis of (2R,3S)-2,3-dihydroxy-3-phenylpropionate wherein the catalyst can be recycled several times with consistent activity.
It is another object of the invention to provide an environmentally safe process for the synthesis of (2R,3S)-2,3-dihydroxy-3-phenylpropionate by avoiding disposal problems.
SUMMARY OF THE INVENTION
Accordingly, the present invention provides a single pot process for the synthesis of taxol side chain comprising (a) synthesizing (2R,3S)-2,3-dihydroxy-3-phenylpropionate by in tandem or simultaneous Heck coupling of bromobenzene and methyl acrylate and N-oxidation of an amine in the presence of a cinchona alkaloid and using a recyclable multifunctional catalyst of the formula IE-IE-PdOsW, wherein IE is an ion-exchanger selected from the group consisting of LDH, quaternary ammonium salt anchored on silica, clay, alumina, magnesia and resin, (b) converting the diol obtained in step (a) without further crystallization into bromoacetate, (c) reacting the bromoacetate with NaN
3
in an organic solvent followed by deacetylation with NaOAc in another organic solvent to obtain an azido alcohol (d) subjecting the azido alcohol to benzoylation followed by hydrogenation to obtain the (2R,3S)-(N-)-benzoyl-3-phenylisoserine methyl ester.
In one embodiment of the invention, ratio of bromobenzene to methyl acrylate is 1:1.
In another embodiment of the invention, the organic solvent used in step (c) for reacting the bromoacetate with NaN
3
is DMF and the organic solvent used for deacetylation is MeOH.
In another embodiment of the invention, the selectivity of diol in step (a) is over 99%.
In a further embodiment of the invention, the multifunctional catalyst is recovered by filtration and reused for several cycles with consistent activity.
In another embodiment of the invention, the solvent selected for the multicomponent reaction is water, acetone, acetonitrile and t-butanol.
In another embodiment of the invention, the cinchona alkaloid comprises a chiral ligand selected from the group consisting of (DHQ)
2
PHAL, (DHQD)
2
PYR, (DHQD)
2
AQN, DHQD-OAc, DHQD-CLB, DHQD-PHN, DHQD-MEQ, DHQD-IND and pseudoenantiomeric forms thereof.
In still another embodiment of the invention, the reactions are effected at a temperature in the range of −20 to +200° C. for a period 0.5 to 48 h.
In another embodiment of the invention, the base used for Heck-coupling is selected from triethylamine, tributylamine, potassium fluoride, and potassium acetate.
In yet another embodiment of the invention, amine used for N-oxidation is selected from the group consisting of N-methyl morpholine, trimethylamine and triethylamine.
In yet another embodiment of the invention, the quantity of multifunctional catalysts used in the reaction is 0.01 to 10 mol % of active species with respect to the substrate.
In another embodiment of the invention, the Heck coupling of bromobenzene and methyl acrylate and the in tandem or simultaneous N-oxidation of an amine is carried out with hydrogen peroxide in the presence of a cinchona alkaloid compound and in a solvent selected from the group consisting of water, acetone, acetonitrile and t-butanol and at a temperature in the range of −20 to +200° C. for a time period in the range of 0.5 to 48 h.
In an embodiment of invention, active species in catalyst ranges between 5 to 30%.
In an embodiment of the present invention, a process for the preparation of taxol side chain that comprises the synthesis of (2R,3S)-2,3-dihydroxy-3-phenylpropionate with greater
Choudary Boyapati M.
Chowdari Naidu S.
Karangula Jyothi
Madhi Sateesh
Mannepalli Lakshmi K.
Council of Scientific and Industrial Research
Nixon & Vanderhye P.C.
Solola Taofiq
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