Process for preparing sulfides and amino acids labeled with sulf

Details Process for preparing sulfides and amino acids labeled with sulf Process for preparing sulfides and amino acids labeled with sulf

1992-06-29

1994-09-06

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435113, 424 9, 562557, 562559, C12P 1304, C12P 1312

Patent

active

053447670

ABSTRACT:
A method of preparing a high specificity .sup.35 S-sulfide comprises preparing a reducing mixture comprising hydrochloric acid, hydriodic acid and hypophosphorus acid, substantially removing any sulfate present in the mixture, admixing the reducing mixture with a composition comprising an .sup.35 S-sulfate corresponding to a desired .sup.35 S-sulfide to obtain the .sup.35 S-sulfide, and recovering the .sup.35 S-sulfide from the admixture. The thus prepared .sup.35 S-sulfides are applicable to the synthesis of high specificity .sup.35 S amino acids and derivatives thereof by sulfhydrylation, e.g., in the presence of an enzyme and a .sup.35 S-sulfide or sulfhydric acid and the O-acetylated amino acid. The labeled amino acid may be separated from the reaction mixture by reverse phase chromatography.

REFERENCES:
patent: 2970893 (1961-02-01), Viles
patent: 4649039 (1987-03-01), Garlick et al.
Suarez, C. et al., "Preparation of carrier-free 35SH2 through direct reduction of the 35S04-contained in neutron irradiated KC1" Radiochimica Acta, v6n3113-114 (1966).
Von Erichsen, L. C., et al. "Preparation and analytical control of H2S(35) of high specific activity" Radioisotopy 12:1019-1027 (1971).
Novopashin, V. M., "Reducing of sulphuric acid to hydrogen sulphide-by hydrogen at raised temp. using aluminium-cobalt-molybdenum, catalyst" Soviet Patents Abstracts, Section Ch, Week 8806 (1987).

Affiliated with

Also associated with

Rating

Process for preparing sulfides and amino acids labeled with sulf does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for preparing sulfides and amino acids labeled with sulf, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for preparing sulfides and amino acids labeled with sulf will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1329213