Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Reexamination Certificate
2011-04-05
2011-04-05
Saucier, Sandra (Department: 1651)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
C435S183000, C435S006120, C435S091200, C435S195000, C435S975000, C536S001110
Reexamination Certificate
active
07919294
ABSTRACT:
The invention relates to a method consisting of bringing into contact, in one container, (a) an aqueous solution of a reaction mixture comprising at least one enzyme, and (b) an aqueous solution of a stabilizing mixture comprising (i) at least one protective agent against drying, (ii) at least one inhibitor of the condensation reaction between carbonyl or carboxyl groups and amine or phosphate groups, and (iii) at least one inert polymer capable of generating a mesh structure preventing the mobility of the dried reagents. The invention also consists of removing all or part of the water contained in the resulting aqueous solution. Said process is suitable for carrying out enzymatic reactions, for example, amplifying, sequencing and characterizing nucleic acids, performing hybridization tests and for the restriction analysis.
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Franco De Sarabia Rosado Pedro Manuel
Limones Lopez Gemma
Madejon Seiz Antonio
Marin Alberdi Maria Dolores
Biotools Biotechnological & Medical Laboratories, S.A.
Hanley Susan
Saucier Sandra
Sughrue & Mion, PLLC
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