Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide
Reexamination Certificate
2003-12-15
2010-10-12
Allen, Marianne P (Department: 1647)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using a micro-organism to make a protein or polypeptide
C530S412000, C435S069100, C435S252330
Reexamination Certificate
active
07811793
ABSTRACT:
A process for preparing a purified refolded monomer or dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to c) in sequence:a) introducing a polynucleotide encoding a bone morphogenetic factor into a bacterium, expressing said bone morphogenetic factor in the form of an inclusion body, recovering said inclusion body and treating it with a denaturing agent to obtain a solubilized monomer,b) treating the solubilized monomer without purification directly with a refolding solution to obtain a refolded monomeric bone morphogenetic factor,c) subjecting the refolded monomeric bone morphogenetic factor to purification.
REFERENCES:
patent: 5399677 (1995-03-01), Wolfman et al.
patent: 5807713 (1998-09-01), Hötten et al.
patent: 5840518 (1998-11-01), Morishita et al.
patent: 6120760 (2000-09-01), Hötten et al.
patent: 6162257 (2000-12-01), Gustilo et al.
patent: 6171584 (2001-01-01), Hötten et al.
patent: 6197550 (2001-03-01), Hötten et al.
patent: 6531450 (2003-03-01), Hötten et al.
patent: 6551801 (2003-04-01), Andou et al.
patent: 6972321 (2005-12-01), Hotten et al.
patent: 0433225 (1991-06-01), None
patent: 1074620 (2001-02-01), None
patent: WO 97/04095 (1997-02-01), None
patent: WO 98/29559 (1998-07-01), None
patent: WO 99/66060 (1999-12-01), None
patent: WO 00/21998 (2000-04-01), None
Andou Hidetoshi
Bechtold Rolf
Honda Jun
Hötten Gertrud
Pohl Jens
Allen Marianne P
Biopharma Gesellschaft zur Biotechnologischen Entwicklung von Ph
Rothwell Figg Ernst & Manbeck P.C.
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