Process for preparing polynucleotides

Details Process for preparing polynucleotides Process for preparing polynucleotides

1988-11-01

1990-05-22

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435814, 435815, 435259, 530412, 530415, 530416, 530417, C12P 1934, C07K 312, C07K 318, C07K 320

Patent

active

049277553

ABSTRACT:
This invention relates to a process of preparation of nucleotide polymers wherein a lysate of a bacterial culture is passed successively through three columns (ion exchange resin, hydrophobic resin and molecular sieve), whereby there is obtained a substantially pure polynucleotidephosphorylase solution.
Polymerization of this agent leads to non toxic and non pyrogen products.
This invention relates also to products thus obtained and to therapeutic compositions containing the same.

REFERENCES:
patent: 3779867 (1973-12-01), Katoh et al.
patent: 3850749 (1974-11-01), Kaufmann et al.
patent: 3917527 (1975-11-01), Shaltiel
patent: 3935185 (1976-01-01), Hutchinson et al.
patent: 4000098 (1976-12-01), Hofstee
patent: 4006059 (1977-02-01), Butler
patent: 4075195 (1978-02-01), Roland
patent: 4379843 (1983-04-01), Cashion et al.
patent: 4617376 (1986-10-01), Maskalick et al.
patent: 4771128 (1988-09-01), Ferris et al.
Cashion et al., Nuc. Acids Res. 8:1167-1185 (1980).

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