Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2007-02-27
2007-02-27
Prouty, Rebecca (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S252330, C435S232000, C435S320100, C536S023200
Reexamination Certificate
active
10662358
ABSTRACT:
The present invention relates to a method for producing middle-chain-length polyhydroxyalkanoate (MCL-PHA) using a maoC gene. The producing method of MCL-PHA according to the present invention comprises the steps of transforming a microorganism with the maoC gene to give a transformant, the microorganism being deleted of a fadB gene and containing a PHA synthase gene; culturing the transformant in medium containing a C6-10carbon source; and obtaining PHA consisting of monomers with 6–10 carbon atoms. When the maoC gene whose function has not yet been established is used according to the present invention, high quality PHA with a higher number of carbon atoms than the prior PHA can be produced at a higher efficiency.
REFERENCES:
patent: 6143952 (2000-11-01), Srienc et al.
patent: WO 98/54329 (1998-12-01), None
patent: WO 99/61624 (1999-12-01), None
patent: WO 01/55436 (2001-08-01), None
Ferrandez A. et al. Catabolism of Phenylacetic acid inEscherichia coli, J. Biol. Chem. 1998, 273, 25974-25986.
Tsuge et al. Molecular cloning of two (R)-specific enoyl-CoA hydratase genes fromPseudomonas aeruginosaand their use for polyhydroxyalkanoate synthesis, FEMS Microbiology Letters, 1999, 189, 193-198.
Agnihotri G. et al. Enoyl-CoA Hydratase: Reaction, Mechanism, and Inhibition, Bioorganic and Medicinal Chemistry, 2003, 11, 9-20.
Park S. et al, Identiification and Characterization of a New Enoyl Coenzyme A Hydratase Involved in Biosynthesis of Medium—Chain—Length Polyhydroxyalkanoates in RecombinantEscherichai coli, J. Bacteriol. 2003, 185, 5391-5397.
Park S. et al. Metabolic engineering ofEscherichia colifor the production of medium-chain—length polyhydroxyalkanoates rich in specific monomers, FEMS Microbiology Letters, 2002, 214, 217-222.
Fukui et al., “Expression and Characterization of (R)-Specific Enoyl Coenzyme A Hydratase Involved in Polyhydroxyalkanoate Biosynthesis byAeromonas caviae,” Journal of Bacteriology, vol. 180, No. 3, (Feb. 1998), pp. 667-673.
Tsuge et al., “Molecular cloning of two (R)-specific enoyl-CoA hydratase genes fromPseudomonas aeruginosaand their use for polyhydroxyalkanoate synthesis,”FEMS Microbiology Letters 184, (1999), pp. 193-198.
Taguchi et al., “Co-expression of 3-ketoacyl-ACP reductase and polyhydroxyalkanoate synthase genes induced PHA production inEscherichia coliHB101 strain,”FEMS Microbiology Letters 176, (1999), pp. 183-190.
Ren et al., “FabG, an NADPH-Dependent 3-Ketoacyl Reductase ofPseudomonas aeruginosa, Provides Precursors for Medium-Chain-Length Poly-3-Hydroxyalkanoate Biosynthesis inEscherichia coli,” Journal of Bacteriology, vol. 182, No. 10, (May 2000), pp. 2978-2981.
Park et al., “Metabolic engineering ofEscherichia colifor the production of medium-chain-length polyhydroxyalkanoates rich in specific monomers,”FEMS Microbiology Letters 214, (2002), pp. 217-222.
Qi et al., “Synthesis of poly(3-hydroxyalkanoates) inEscherichia coliexpressing the PHA synthase genephaC2fromPseudomonas aeruginosa: comparison of PhaC1 and PhaC2,”FEMS Microbiology Letters 157, (1997), pp. 155-162.
Qi et al., “Metabolic routing towards polyhydroxyalkanoic acid synthesis in recombinantEscherichia coli(fadR): inhibition of fatty acid β-oxidation by acrylic acid,”FEMS Microbiology Letters 167, (1998), pp. 89-94.
Langenbach et al., “Functional expression of the PHA synthase genephaC1 fromPseudomonas aeruginosainEscherichia coliresults in poly(3-hydroxyalkanoate) synthesis,”FEMS Microbiology Letters 150, (1997), pp. 303-309.
Snell et al., “YfcX Enables Medium-Chain-Length Poly(3-Hydroxyalkanoate) Formation from Fatty Acids in RecombinantEscherichia coli fadBStrains,”Journal of Bacteriology, Oct. 2002, pp. 5696-5705.
Steinebach et al., “Cloning of themaoAgene that encodes aromatic amine oxidase ofEscherichia coliW3350 and characterization of the overexpressed enzyme,”Eur. J. Biochem., vol. 237, (1996), pp. 584-591.
Blattner et al., “The Complete Genome Sequence ofEscherichia coliK-12,”Science, vol. 277, (Sep. 5, 1997), pp. 1453-1462.
Jeong et al., “Excretion of Human β-Endorphin into Culture Medium by Using Outer Membrane Protein F as a Fusion Partner in RecombinantEscherichia coli,” Applied and Environmental Microbiology, (vol. 68, No. 10, (Oct. 2002), pp. 4979-4985.
Park et al., “Enrichment of specific monomer in medium-chain-length poly(3-hydroxyalkanoates) by amplification offadDandfadEgenes in recombinantEscherichia coli,” Enzyme and Microbial Technology, vol. 33, (2003), pp. 62-70.
Matsusaki et al., “Cloning and Molecular Analysis of the Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) Biosynthesis Genes inPseudomonassp. Strain 61-3,”Journal of Bacteriology, vol. 180, No. 24, (Dec. 1998), pp. 6459-6467.
Peekhaus et al., “Positive and Negative Transcriptional Regulation of theEscherichia coliGluconate Regulation GenegntTby GntR and the Cyclic AMP (cAMP)-cAMP Receptor Protein Complex,”Journal of Bacteriology, vol. 180, No. 7, (Apr. 1998), pp. 1777-1785.
Sambrook et al., “Molecular Cloning, Second Edition, A Laboratory Manual,”Cold Spring Harbor Laboratory, (1989), pp. xi-xxxv.
Kovach et al., “Four new derivatives of the broad-host-range cloning vestor pBBR1 MCS, carrying different antibiotic-resistance cassettes,”Gene, vol. 166, (1995), pp. 175-176.
Lee Sang Yup
Park Si Jae
Korea Advanced Institute of Science and Technology
Prouty Rebecca
Walicka Malgorzata
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