Process for preparing phosphorodiamidites

Details Process for preparing phosphorodiamidites Process for preparing phosphorodiamidites

2007-10-02

2007-10-02

Parsa, J. (Department: 1621)

Organic compounds -- part of the class 532-570 series

Organic compounds

Phosphorus esters

Reexamination Certificate

active

10540160

ABSTRACT:
A method of phosphorodiamidite production comprising the steps of reacting a phosphorus trihalide with a dialkyl amine in a polar solvent to form an intermediate compound. This intermediate compound is then subsequently reacted with an hydroxylalkyl compound and a dialkyl amine in the presence of a non-polar cosolvent. Following filtration to remove the solid by-product the two solvents form separate layers. This is advantageous as the upper, non-polar solvent, layer contains the high-purity phosphorodiamidite product.

REFERENCES:
patent: 2003/0225274 (2003-12-01), Bosch et al.
patent: 2003/0236233 (2003-12-01), Shamblee et al.
patent: 62212395 (1987-09-01), None
patent: WO 03/087130 (2003-10-01), None
patent: WO 03/106468 (2003-12-01), None
Nielsen et al., Improved synthesis of 2-cyanoethyl N, N, N′, N′-tetraisopropylphosphorodiamidite, (Nucleic acids Research (1987), 15 (8), 3626).
Patent Abstracts of Japan, vol. 012 No. 075 (C-480), Mar. 9, 1988, of JP 62 212395 A, (Nippon Zeon Co. Ltd), Sep. 18, 1987.
Hamamoto S. Takaku H: “New approach to the Synthesis of Deoxyribonucleoside Phosphoramidite Derivatives”,Chemistry Letters, Chemical Society of Japan, Tokyo, JP, vol. 8, 1986, pp. 1401-1404, XP002902766.
Pfleiderer W. et al: “Inhibition of HIV-a replication and activation of RNase L by phosphorothioate/phosphodiester 2′,5′-oligoadenylate derivatives”,,Journal of Biological Chemistry, American Society of Biological Chemists, Baltimore, Md., US, vol. 270-, No. 11, Mar. 17, 1995, pp. 5963-5978, XP002079044.
Houalla D, et al.: “Preparations ET Quelques Proprietes DE Composes Contenant LA Liaison Phosphore Trivalent-Azote”,Bulletin de La Societe Chimique de France, Societe Francaise de Chimie, Paris, France, 1965, pp. 2368-2373, pp. 2368, 2370.
Fluka Chemika, Biochemika und Analytika Katalog 1997, Fluka Chmie AG XP002277275, p. 434.

Affiliated with

Also associated with

Rating

Process for preparing phosphorodiamidites does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Process for preparing phosphorodiamidites, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for preparing phosphorodiamidites will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3881670