Process for preparing obesity protein analogs

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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435176, 435177, 435212, 435223, C12P 2102

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058405178

ABSTRACT:
The present invention is directed to an improved process for preparing in high yield an obesity protein analog using a dipeptidylaminopeptidase isolated from the slime mold, Dictyostelium discoideum.

REFERENCES:
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patent: 5614379 (1997-03-01), MacKellar
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Flam, "Obesity Gene Discovery May Help Solve Weighty Problem", Science, 266, 1477-1478 (Dec. 2, 1994).
Chan, et al., "Dipeptidyl-Aminopeptidases and Aminopeptidases in Dictyostelium discoideum", Biochemical and Biophysical Research Communications, 127:3, 962-968 (Mar. 29, 1985).
Chan, et al., "Partial Purification and Characterization of Dipeptidyl-aminopeptidase III from Dictyostelium discoideum", Experimental Mycology, 11, 27-35 (1987).
Hutchinson, et al., "The preparation and properties on immobilised dipeptidyl-aminopeptidase I (cathepsin C)"Biochimica et Biophysica Acta, 916, 1-4 (1987).
Erickson, et al., "Interaction of Purified Brush-Border Membrane Aminopeptidase N and Dipeptidyl Peptidase IV with Lectin-Sepharose Derivatives", Biochimica et Biophysica Acta, 743, 37-42 (1983).
Huang, et al. "The Purification, Specificity, and Role of Dipeptidyl Peptidase III in Dictyostelium discoideum", Experimental Mycology, 16, 102-109 (1992).

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