Process for preparing nontoxic lipopolysaccharides from acidiphi

Details Process for preparing nontoxic lipopolysaccharides from acidiphi Process for preparing nontoxic lipopolysaccharides from acidiphi

1996-12-10

1998-12-08

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435822, 4242341, C12P 1904, A61K 3902

Patent

active

058467890

ABSTRACT:
A process for preparing a nontoxic lipopolysaccharide, which comprises (i) cultivating in a culture medium bacteria of the genus Acidophilium having accession No. MTCC 1979, (ii) separating the culture bacteria, and repeatedly washing the separated bacteria, (iii) extracting the washed bacteria with a lipophilic solvent, (iv) cooling the extract and repeatedly centrifuging the cooled material, (v) dialyzing the supernatant resulting from said centrifuging, (vi) lyophilizing the dialyzed material, (vii) precipitating the lipopolysaccharide by treating the lyophilized material with a polar solvent, and (viii) drying the precipitated polyliposaccharide.

REFERENCES:
patent: 4230801 (1980-10-01), Gutnick et al.
patent: 5382428 (1995-01-01), Livingston-Wheeler et al.
Basu et al., "Lipopolysaccharides of the acidophilic heterotrophic bacteria Acidiphilium cryptum and Acidiphilium symbioticum", Microbiology Letters 118 : 65-70 (1994).

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