Process for preparing multilayer enzyme coating on a fiber

Details Process for preparing multilayer enzyme coating on a fiber Process for preparing multilayer enzyme coating on a fiber

2005-09-30

2009-11-03

Naff, David M (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

C435S041000, C435S176000, C435S177000, C435S180000, C435S283100, C435S287100, C435S289100

Reexamination Certificate

active

07611835

ABSTRACT:
A process for preparing high stability, high activity biocatalytic materials is disclosed and processes for using the same. The process involves coating of a material or fiber with enzymes and enzyme aggregate providing a material or fiber with high biocatalytic activity and stability useful in heterogeneous environments. In one illustrative approach, enzyme “seeds” are covalently attached to polymer nanofibers followed by treatment with a reagent that crosslinks additional enzyme molecules to the seed enzymes forming enzyme aggregates thereby improving biocatalytic activity due to increased enzyme loading and enzyme stability. This approach creates a useful new biocatalytic immobilized enzyme system with potential applications in bioconversion, bioremediation, biosensors, and biofuel cells.

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