Process for preparing mouse interferon

Drug – bio-affecting and body treating compositions – Lymphokine

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435 68, 435811, A61K 4502, A61K 3900

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active

043282075

ABSTRACT:
The present invention relates to a process which is easily applicable for industrial production of mouse interferon.
Particularly, the present invention relates to a process based on the discovery that a large amount and high activity of mouse interferon is obtained by transplanting established mouse tumor cells to other warm-blooded animal body or inoculating the cells in a culture medium charged in a filter-membrane-interposed diffusion chamber which is designed and fitted up to or in the animal body so that its nutrient body fluid feeds the cells, multiplying the transplanted or inoculated cells in the warm-blooded animal body or the diffusion chamber utilizing said body fluid, and exposing the multiplied cells to the action of interferon inducer in vitro or in vivo.

REFERENCES:
patent: 4276282 (1981-06-01), Sugimoto
Chemical Abstracts, vol. 81, p. 391, Abst. No. 167513d, Sans et al., "Production of Mouse Interferon with High Liters in a Large-Scale Suspension Culture", 1974.
Chemical Abstracts, vol. 79, p. 298, Abst. No. 90455k, Tovey et al., "Production of Interferon by Chemostat Cultures of Mouse LS-Cells Grown in Chemically Defined, Protein Free Medium", 1973.
Chemical Abstracts, vol. 74, p. 190, Abst. No. 21415g, Khesin et al., "Cytological Study of a Peritoneal Exudate from Mice in Course of Interferon Production In Vitro", 1971.
Chemical Abstracts, vol. 89, p. 382, Abst. No. 88763x, Zaretsku et al., "Matrix Synthesis of Mouse Interferon in Heterological Systems In Vitro and In Vivo", 1978.
Miyoshi et al., Cancer, vol. 40, No. 6, pp. 2999-3003, 1977.
Dianzani, F. et al., Advances in Experimental Medicine and Biology, vol. 110, pp. 119-131, 1978.
De Maeyer-Guinard, J., "Mouse Leukemia: Depression of Serum Interferon Production", Science, (Sep. 1972), pp. 797-799.
Thompson, J. S. et al., "Heterologous Transplantation of Mouse Tumors into the Newborn Albino Rat", Cancer Research, vol. 20, pp. 1365-1371, (1960).
Solov'ev, V. D. et al., "Interferon Theory and Applications", Plenum Press, New York -London, 1973, pp. 3-18.

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